VEMO 99’s laboratories are staffed with trained chemists and technicians with years of industry knowledge and expertise. Our labs are equipped with high-tech instrumentation, allowing for accurate analysis and testing. Thus, we can provide enzyme activity analysis for a broad range of products, markets and industries.

Enzymes are crucial additives to both animal feeds and human dietary supplements. Their use as digestion and metabolic supporters is becoming highly common in a wide variety of application fields.

Since enzymes are bio active molecules, they are highly sensitive to manufacturing processes, like temperature, pelleting, moisture, etc. Thus, it is important to measure the real enzyme activity in both raw materials and finished products. In addition, since enzymes are biological products, they may reduce their activity with time and storage conditions, making it crucial to develop standardized methods for their analysis and validation. It is also important to ensure that enzyme activity analysis is conducted according to the optimal working conditions for each product, like pH, temperature, co-factors, substrates, etc.

With more than 20 years of experience in the design and manufacturing of active enzymes, our team has broad experience with a number of different enzyme activity assays, providing for the high quality of VEMO feed and food enzymes. Thus, we transfer our professional knowledge into assisting you to meet your R&D and business goals. VEMO scientists are confident in providing the comprehensive services for enzyme research. We offer versatile testing methods of measuring the enzyme activity and many enzymes can be assayed in custom ways, upon your request.

Enzyme Analysis we provide

  • Neutral Protease

    Hydrolysis of casein @ pH 7.0 and 30°C (UV-VIS spectroscopy)

  • Neutral Protease

    Hydrolysis of casein @ pH 7.0 and 37°C (UV-VIS spectroscopy)

  • Acid Protease

    Hydrolysis of casein @ pH 2.6 and 30°C (UV-VIS spectroscopy)

  • Protease HUT

    Hydrolysis of hemoglobin @ pH 4.7 and 40°C (UV-VIS spectroscopy)

  • β-Glucanase

    Hydrolysis of β-glucan @ pH 6.8 and 45°C (UV-VIS spectroscopy)

  • Cellulase

    Hydrolysis of Na-CMC (carboxymethyl cellulose- sodium salt) @ pH 5.0 and 50°C (UV-VIS spectroscopy)

  • α-Galactosidase

    Hydrolysis of p-nitrophenyl-α-D-galactopyranoside @ pH 5.5 and 37°C (UV-VIS spectroscopy)

  • Xylanase

    Hydrolysis of AZCL-wheat arabinoxylan @ pH 4.7 and 40°C (UV-VIS spectroscopy)

  • Xylanase

    Hydrolysis of AZCL-wheat arabinoxylan @ pH 4.7 and 50°C (UV-VIS spectroscopy)

  • Xylanase BXU

    Hydrolysis of birch xylan @ pH 5.3 and 50°C (UV-VIS spectroscopy)

  • Phytase PPU

    Hydrolysis of sodium phytate @ pH 5.0 and 37°C (UV-VIS spectroscopy)

  • Phytase FTU

    Hydrolysis of sodium phytate @ pH 5.5 and 37°C (UV-VIS spectroscopy)

  • Mannanase

    Hydrolysis of AZCL-galactomannan @ pH 4.0 and 40°C (UV-VIS spectroscopy)

  • Lipase FIP

    Hydrolysis of olive oil @ pH 7.0 and 37°C (UV-VIS spectroscopy)

  • Phospholipase

    Hydrolysis of L-α-phosphatidylcholine @ pH 7.0 and 37°C (UV-VIS spectroscopy)

  • Bacterial Amylase

    Hydrolysis of soluble starch @ pH 6.0 and 30°C (UV-VIS spectroscopy)

  • Fungal Amylase

    Hydrolysis of soluble starch @ pH 4.7 and 30°C (UV-VIS spectroscopy)

  • Fungal Amylase

    Hydrolysis of soluble starch @ pH 4.7 and 30°C (UV-VIS spectroscopy)


Consultation and Analysis (for any samples of VEMO’s enzymes clients)


Enzyme activity analysis from the list above, depending on type


our instrumentation

  • UltiMate 3000 HPLC (Thermo Scientific) – High Performance Liquid Chromatography is considered one of the most powerful tools in analytical chemistry used to separate, identify, and quantify compounds in a variety of materials. We are applying this technique for determination and quantification of a wide variety of substances in raw materials, like botanicals, extracts and end-customer dietary supplements, testing for concentration and purity;
  • Spectramax 190 Microplate Reader (Molecular Devices) – For determination of enzyme activity, while measuring absorption, fluorescence, luminescence; detection of antibiotics in enzymes
  • LAMBDA 365 Spectrophotometer UV Express – Our lab uses UV/VIS spectroscopy for measuring enzyme kinetics, solvent analysis, quantification of DNA and proteins, USP method compliance. It is also used to quantify organic compounds in natural products and dietary supplements. Working modes: photometric, spectral, quantifying, kinetic, multicomponent quantifying mode – quantification of up to 8 components at a time;
  • Drying Oven Kenton GX-40/70/130 – Used in drying process, sterilization, thermostatic storage, heat treatment and other fields;
  • pH Meters – 913 Metrohm; PHM – 80/82 (Radiometer A/C);
  • pH Electrodes – Electrode, Viscotrode;
  • Table Top Centrifuge TDL-40B;
  • HY-200 Lab Test Sieve For Particle Size Analysis – Separation of particles with mesh sizes from 1.18 mm to 0.125 mm, allowing for accurate sampling and analyzing of different materials;
  • Moisture Analyzer METTLER TOLEDO HE73 – For determining the moisture content of wide variety of products to maintain quality and ensure optimum shelf-life. Halogen heater with temperature range of 50 – 200oС. Result accuracy – 0.01%;
  • Environmental Test Chamber KG-150 – For product stability studies and climatic tests, generating exceptional control and uniformity of both temperature and humidity;
  • Density Tester BT-301 – For determination of bulk powder density. The instrument is in accordance with the national standards GB/T21354-2008 and the USP;
  • Precision Balance METTLER TOLEDO ME2002T – 2200 g capacity, 0.01 g readability;
  • Analytical Balance METTLER TOLEDO ME204T – 220 g capacity, 0.1 mg readability;
  • Laboratory Vacuum Pump (KFN LABOPORT®) – Filter mesh 0.45 µm and diameter 47 mm;